N-Ras (mutated Q61R) Recombinant Rabbit mAb (S-R155)

GTPase NRas,Transforming protein N-Ras

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0505

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:5000	Hu
IHC-P	1:500	Hu

背景介绍

All mammalian cells express 3 closely related Ras proteins, termed H-Ras, K-Ras, and N-Ras, that promote oncogenesis when they are mutationally activated at codon 12, 13, or 61. N-Ras mutations were more strongly associated with hematopoietic tumors.

免疫印迹
- WB result of N-Ras (mutated Q61R) Rabbit mAb
  Primary antibody: N-Ras (mutated Q61R) Rabbit mAb at 1/5000 dilution
  Lane 1: SK-MEL-2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 21 kDa
  Observed MW: 20 kDa
- WB result of N-Ras (mutated Q61R) Rabbit mAb
  Primary antibody: N-Ras (mutated Q61R) Rabbit mAb at 1/5000 dilution
  Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
  Lane 2: 293T transfected with N-Ras-Myc-His fusion protein whole cell lysate 20 µg
  Lane 3: 293T transfected with N-Ras (mutated Q61R)-Myc-His fusion protein whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 25 kDa
  Observed MW: 25 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human melanoma. Anti-N-Ras (mutated Q61R) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.