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Rabbit分子别名
Renal glomerulus-specific cell adhesion receptor免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9QZS7克隆号
S-543-63抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. The renal filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane, and the podocytes of epithelial cells.
免疫印迹
WB result of Nephrin Rabbit mAb
Primary antibody: Nephrin Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 200 kDa
(This blot was developed with high sensitivity substrate)
WB result of Nephrin Rabbit mAb
Primary antibody: Nephrin Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 200 kDa
Exposure time: 180 s (This blot was developed with high sensitivity substrate)
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Nephrin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Nephrin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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