Nephrin Recombinant Rabbit mAb (S-543-63)

Renal glomerulus-specific cell adhesion receptor

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0504

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IHC-P	1:500	Hu, Rt

背景介绍

Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. The renal filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane, and the podocytes of epithelial cells.

免疫印迹
- WB result of Nephrin Rabbit mAb
  Primary antibody: Nephrin Rabbit mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Lane 2: mouse kidney lysate 20 µg
  Negative control: mouse spleen lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 137 kDa
  Observed MW: 200 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of Nephrin Rabbit mAb
  Primary antibody: Nephrin Rabbit mAb at 1/1000 dilution
  Lane 1: rat kidney lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 137 kDa
  Observed MW: 200 kDa
  Exposure time: 180 s (This blot was developed with high sensitivity substrate)
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Nephrin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-Nephrin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.