FUBP1 Recombinant Rabbit mAb (S-739-21)

Far upstream element-binding protein 1,FBP,FUSE-binding protein 1,DNA helicase V (hDH V)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0502

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

FUBP1
分子别名

Far upstream element-binding protein 1, FBP, FUSE-binding protein 1, DNA helicase V (hDH V)
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q96AE4
克隆号

S-739-21
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Rt
IHC-P 1:2000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Rt
IHC-P	1:2000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

FUBP1 is a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

免疫印迹
- WB result of FUBP1 Rabbit mAb
  Primary antibody: FUBP1 Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 10 µg
  Lane 2: HeLa whole cell lysate 10 µg
  Lane 3: HepG2 whole cell lysate 10 µg
  Lane 4: K-562 whole cell lysate 10 µg
  Lane 5: Raji whole cell lysate 10 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 74 kDa
- WB result of FUBP1 Rabbit mAb
  Primary antibody: FUBP1 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW 264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 74 kDa
- WB result of FUBP1 Rabbit mAb
  Primary antibody: FUBP1 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 74 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling FUBP1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- FUBP1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating FUBP1 in 0.4 mg C6 whole cell lysate.
  Western blot was performed on the immunoprecipitate using FUBP1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: C6 whole cell lysate 10 µg (Input)
  Lane 2: FUBP1 Rabbit mAb IP in C6 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in C6 whole cell lysate
  Predicted MW: 68 kDa
  Observed MW: 74 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-FUBP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-FUBP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).