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CD82 Recombinant Rabbit mAb (S-673-44)

CD82 antigen,C33 antigen,IA4,Inducible membrane protein R2,Metastasis suppressor Kangai-1,Suppressor of tumorigenicity 6 protein,Tetraspanin-27 (Tspan-27),KAI1,SAR2,ST6,TSPAN27

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0500

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27 (Tspan-27), KAI1, SAR2, ST6, TSPAN27
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P27701
克隆号

S-673-44
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:500 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC-P	1:500	Hu, Ms, Rt

背景介绍

CD82 is a membrane glycoprotein that is a member of the tetraspanin/transmembrane 4 superfamily. Expression of this protein has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients.

免疫印迹
- WB result of CD82 Rabbit mAb
  Primary antibody: CD82 Rabbit mAb at 1/1000 dilution
  Lane 1: SK-OV-3 whole cell lysate 20 µg
  Lane 2: Raji whole cell lysate 20 µg
  Lane 3: U-87 MG whole cell lysate 20 µg
  Lane 4: SW480 whole cell lysate 20 µg
  Lane 5: BxPC-3 whole cell lysate 20 µg
  Lane 6: Jurkat whole cell lysate 20 µg
  Low expression control: SK-OV-3 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 30~60 kDa
- WB result of CD82 Rabbit mAb
  Primary antibody: CD82 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 38~50 kDa
- WB result of CD82 Rabbit mAb
  Primary antibody: CD82 Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 40~50 kDa
免疫沉淀
- CD82 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD82 in 0.4 mg U-87 MG whole cell lysate.
  Western blot was performed on the immunoprecipitate using CD82 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: U-87 MG whole cell lysate 10 µg (Input)
  Lane 2: CD82 Rabbit mAb IP in U-87 MG whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in U-87 MG whole cell lysate
  Predicted MW: 30 kDa
  Observed MW: 30~60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD82 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-CD82 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD82 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CD82 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CD82 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti-CD82 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.