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CaMKII-α/β Recombinant Rabbit mAb (S-R329)

Calcium/calmodulin-dependent protein kinase type II subunit alpha,CaM kinase II subunit alpha,CaMK-II subunit alpha,CAMK2A,CAMKA,KIAA0968,Calcium/calmodulin-dependent protein kinase type II subunit beta,CaM kinase II subunit beta,CAMK2B,CAM2,CAMK2,CAMKB

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0496

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968,Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CAMK2B; CAM2; CAMK2; CAMKB
细胞定位

Synapse, Cell projection
Accession

Q9UQM7
克隆号

S-R329
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IHC-P 1:100 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IHC-P	1:100	Ms, Rt

背景介绍

CaMKII-α is an enzyme that belongs to the serine/threonine-specific protein kinase family, as well as the Ca2+/calmodulin-dependent protein kinase II subfamily. Ca2+ signaling is crucial for several aspects of synaptic plasticity at glutamatergic synapses. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity.

免疫印迹
- WB result of CaMKII-α Rabbit mAb
  Primary antibody: CaMKII-α Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 50, 60 kDa
- WB result of CaMKII-α Rabbit mAb
  Primary antibody: CaMKII-α Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 50, 60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CaMKII-α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CaMKII-αantibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.