TMPRSS2 Recombinant Rabbit mAb (S-735-40)

Transmembrane protease serine 2,Serine protease 10,PRSS10

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0491

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Transmembrane protease serine 2, Serine protease 10, PRSS10
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

O15393
克隆号

S-735-40
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:500
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:500
IP	1:50

背景介绍

TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a low density lipoprotein receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This protein is up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. TMPRSS2 participates in proteolytic cascades necessary for normal physiological function of the prostate.

免疫印迹
- WB result of TMPRSS2 Rabbit mAb
  Primary antibody: TMPRSS2 Rabbit mAb at 1/1000 dilution
  Lane 1: LNCaP whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 22, 55 kDa
  (This blot was developed with high sensitivity substrate)
免疫沉淀
- TMPRSS2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating TMPRSS2 in 0.4 mg LnCap whole cell lysate.
  Western blot was performed on the immunoprecipitate using TMPRSS2 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: LnCap whole cell lysate 20 µg (Input)
  Lane 2: TMPRSS2 Rabbit mAb IP in LnCap whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in LnCap whole cell lysate
  Predicted MW: 54 kDa
  Observed MW: 22, 55 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-TMPRSS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TMPRSS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-TMPRSS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.