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宿主来源
Rabbit分子别名
Slit homolog 2 protein, Slit-2, SLIL3免疫原
Synthetic Peptide细胞定位
SecretedAccession
O94813克隆号
S-730-41抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:100 Ms, Rt
Slit guidance ligand 2 (Slit-2) is a new type of adipoprotein, and the full-length Slit-2 protein is a secretory ligand. This protein splits into two fragments, a 140 kDa N-terminal product (Slit-2-N) and a 50–60 kDa C-terminal product (Slit-2-C), and interacts with Robo receptors. Robo receptors are divided into Robo1, Robo2, Robo3 and Robo4, and the binding of Slit-2 to specific Robo receptors regulates specific cell functions. Slit-2 has been reported to play an important role in neuronal and vascular development. Slit-2 is also involved in the development of many organs and is related to cancer apoptosis, migration, invasion, occurrence and development. In addition, Slit-2 can regulate different inflammatory diseases and inflammatory phenotypes and then determine the activity and severity of the disease.
免疫印迹
WB result of SLIT2 Rabbit mAb
Primary antibody: SLIT2 Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Negative control: T-47D whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 170 kDa
Observed MW: 200 kDa
(This blot was developed with high sensitivity substrate)
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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