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ALOX15 Recombinant Rabbit mAb (S-R308)

Polyunsaturated fatty acid lipoxygenase ALOX15,12/15-lipoxygenase,Leukocyte-type arachidonate 12-lipoxygenase (12-LOX),Arachidonate 15-lipoxygenase (15-LOX,15-LOX-1),Arachidonate omega-6 lipoxygenase,Hepoxilin A3 synthase Alox15,Linoleate 13S-lipoxygenase,LOG15

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0482

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, Leukocyte-type arachidonate 12-lipoxygenase (12-LOX), Arachidonate 15-lipoxygenase (15-LOX; 15-LOX-1), Arachidonate omega-6 lipoxygenase, Hepoxilin A3 synthase Alox15, Linoleate 13S-lipoxygenase, LOG15
细胞定位

Cytoplasm, Cell membrane
Accession

P16050
克隆号

S-R308
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
ICC 1:500
ICFCM 1:500

应用	稀释度
WB	1:1000
ICC	1:500
ICFCM	1:500

背景介绍

ALOX15 (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal enzyme in the metabolism of polyunsaturated fatty acids to a wide range of physiologically and pathologically important products. By metabolizing the ω-3 polyunsaturated fatty acids, eicosapentaenoic acid and docosahexaenoic acid, into 17-HpDHA, 17-HDHA, and the resolvins and protectins, ALOX15's metabolic action is thought to be one mechanism by which dietary ω-3 polyunsaturated fatty acids, particularly fish oil, act to ameliorate inflammation, inflammation-related diseases, and certain cancers.

免疫印迹
- WB result of ALOX15 Rabbit mAb
  Primary antibody: ALOX15 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 75 kDa
  Observed MW: 75 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling ALOX15 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-ALOX15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).