ALOX15 Recombinant Rabbit mAb (S-R308)
Polyunsaturated fatty acid lipoxygenase ALOX15,12/15-lipoxygenase,Leukocyte-type arachidonate 12-lipoxygenase (12-LOX),Arachidonate 15-lipoxygenase (15-LOX,15-LOX-1),Arachidonate omega-6 lipoxygenase,Hepoxilin A3 synthase Alox15,Linoleate 13S-lipoxygenase,LOG15
货号： S0B0482
- 价格： ￥600
- 规格：
- 数量：
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宿主来源
Rabbit分子别名
Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, Leukocyte-type arachidonate 12-lipoxygenase (12-LOX), Arachidonate 15-lipoxygenase (15-LOX; 15-LOX-1), Arachidonate omega-6 lipoxygenase, Hepoxilin A3 synthase Alox15, Linoleate 13S-lipoxygenase, LOG15细胞定位
Cell membrane, CytoplasmAccession
P16050克隆号
S-R308抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:500 |
| ICFCM | 1:500 |
ALOX15 (also termed arachidonate 15-lipoxygenase, 15-lipoxygenase-1, 15-LO-1, 15-LOX-1) is, like other lipoxygenases, a seminal enzyme in the metabolism of polyunsaturated fatty acids to a wide range of physiologically and pathologically important products. By metabolizing the ω-3 polyunsaturated fatty acids, eicosapentaenoic acid and docosahexaenoic acid, into 17-HpDHA, 17-HDHA, and the resolvins and protectins, ALOX15's metabolic action is thought to be one mechanism by which dietary ω-3 polyunsaturated fatty acids, particularly fish oil, act to ameliorate inflammation, inflammation-related diseases, and certain cancers.
免疫印迹
WB result of ALOX15 Rabbit mAb
Primary antibody: ALOX15 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 75 kDa
(This blot was developed with high sensitivity substrate)
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling ALOX15 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-ALOX15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
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