Insulin Recombinant Rabbit mAb (S-638-19)

INS

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0465

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:1000	Ms
IHC-P	1:2000	Hu, Ms, Rt

背景介绍

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the INS gene. It is considered to be the main anabolic hormone of the body. It regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. Proinsulin is cleaved into insulin and C-peptide. The two are stored in the secretory granules of the pancreatic β-cells and eventually released together in equimolar amounts. C-peptide has an important role in facilitating the correct folding of insulin and formation of its disulfide bridges.

免疫印迹
- WB result of C-Peptide Rabbit mAb
  Primary antibody: C-Peptide Rabbit mAb at 1/1000 dilution
  Lane 1: mouse skeletal muscle lysate 20 µg
  Lane 2: mouse pancreas lysate 20 µg
  Negative control: mouse skeletal muscle lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 9.4, 12 kDa
  Observed MW: 12, 13 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat pancreas. Anti-C-Peptide antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.