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SMAD3 Recombinant Rabbit mAb (S-517-122)

Mothers against decapentaplegic homolog 3,MAD homolog 3,Mad3,Mothers against DPP homolog 3,hMAD-3,JV15-2,SMAD family member 3,MADH3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0461

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

SMAD3
分子别名

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; MADH3
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P84022
克隆号

S-517-122
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ICFCM ,
ChIP ,
IHC-P ? ,
ICC ? ,
WB
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Av, Dr
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:100
ICC	1:100
ChIP	1:50

背景介绍

SMAD3 is a member of the SMAD family of proteins. It acts as a mediator of the signals initiated by the transforming growth factor beta (TGF-β) superfamily of cytokines, which regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

免疫印迹
- WB result of SMAD3 Rabbit mAb
  Primary antibody: SMAD3 Rabbit mAb at 1/1000 dilution
  Lane 1: HT-1080 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: HCT116 whole cell lysate 20 µg
  Lane 4: HT-29 whole cell lysate 20 µg
  Lane 5: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 50 kDa
  Exposure time: 60 s (This blot was developed with high sensitivity substrate)
- WB result of SMAD3 Rabbit mAb
  Primary antibody: SMAD3 Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 50 kDa
  Exposure time: 60 s (This blot was developed with high sensitivity substrate)
- WB result of SMAD3 Rabbit mAb
  Primary antibody: SMAD3 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 50 kDa
  Exposure time: 60 s (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SMAD3 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-SMAD3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMAD3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SMAD3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SMAD3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human transitional cell cancer. Anti-SMAD3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-SMAD3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HaCaT + TGF-β (7ng/mL, 1h) cells cross - linked with 1% formaldehyde for 10min, then chromatin was fragmented by sonication. Parallel reactions used SMAD3Recombinant Rabbit mAb (S-517-122) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of ID1, CDKN1A and SAT-α in
  SMAD3 Recombinant Rabbit mAb (S-517-122)
  -immunoprecipitated sample.