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Rabbit抗原名称
EGFR (delE746-A750)分子别名
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P00533克隆号
S-451-168抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,ICC ? ,WB ,IP稀释度
应用 稀释度 WB 1:1000 IP 1:50 IHC-P 1:500 ICC 1:500
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. The EGFR is essential for ductal development of the mammary glands, and agonists of the EGFR such as amphiregulin, TGF-α, and heregulin induce both ductal and lobuloalveolar development even in the absence of estrogen and progesterone. EGFR mutations, del E746-A750 in exon 19 and L858R in exon 21 in tumor cells of NMLC represent biomarkers of response to tyrosine kinase inhibitors (TKI) therapy. Patients with tumors positive for EGFR mutations show better response and greater survival. These mutations occupy 90% of mutations in lung cancer.
免疫印迹
WB result of EGFR (delE746-A750) Rabbit mAb
Primary antibody: EGFR (delE746-A750) Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: H1975 whole cell lysate 20 µg
Lane 4: HCC827 whole cell lysate 20 µg
Negative control: A431 whole cell lysate; A549 whole cell lysate; H1975 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 175 kDa
免疫沉淀
EGFR (delE746-A750) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating EGFR (delE746-A750) in 0.4 mg HCC827 whole cell lysate.
Western blot was performed on the immunoprecipitate using EGFR (delE746-A750) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HCC827 whole cell lysate 20 µg (Input)
Lane 2: EGFR (delE746-A750) Rabbit mAb IP in HCC827 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HCC827 whole cell lysate
Predicted MW: 134 kDa
Observed MW: 175 kDa
免疫组化
IHC shows positive staining in paraffin-embedded HCC827 cells and negative staining in paraffin-embedded A549, H1975 and A431 cells. Anti-EGFR (delE746-A750) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-EGFR (delE746-A750) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HCC827 cells. Anti-EGFR(delE746_A750) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).
Negative control：ICC shows negative staining in A431 cells. Anti-EGFR(delE746_A750) antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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