p27 Recombinant Rabbit mAb (S-141-135)

Cyclin-dependent kinase inhibitor 1B,Cyclin-dependent kinase inhibitor p27,p27Kip1,Cdkn1b,KIP-1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0444

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b, KIP-1
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Nucleus
Accession

P46414
克隆号

S-141-135
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IP 1:50 Ms
IHC-P 1:500 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IP	1:50	Ms
IHC-P	1:500	Ms, Rt

背景介绍

P27 is a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. It binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle. p27 is considered a tumor suppressor because of its function as a regulator of the cell cycle. In cancers it is often inactivated via impaired synthesis, accelerated degradation, or mislocalization. p27 can also be mislocalized to the cytoplasm in order to facilitate metastasis.

免疫印迹
- WB result of p27 Rabbit mAb
  Primary antibody: p27 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: Raw 264.7 whole cell lysate 20 µg
  Lane 3: mouse thymus lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 24 kDa
- WB result of p27 Rabbit mAb
  Primary antibody: p27 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat thymus lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 25 kDa
免疫沉淀
- p27 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p27 in 0.4 mg NIH/3T3 whole cell lysate.
  Western blot was performed on the immunoprecipitate using p27 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: NIH/3T3 whole cell lysate 20 µg (Input)
  Lane 2: p27 Rabbit mAb IP in NIH/3T3 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 whole cell lysate
  Predicted MW: 22 kDa
  Observed MW: 25 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-p27 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse thymus. Anti-p27 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse lung. Anti-p27 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-p27 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-p27 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-p27 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.