CD28 Mouse mAb (S-714-76)

T-cell-specific surface glycoprotein CD28,TP44

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0442

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Mouse
抗原名称

CD28
分子别名

T-cell-specific surface glycoprotein CD28, TP44
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P10747
克隆号

S-714-76
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

1.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?
稀释度

应用稀释度

FCM 1:1500
ICC 1:500

应用	稀释度
FCM	1:1500
ICC	1:500

背景介绍

CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.

流式分析
- Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD28 antibody at 1/1500 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: THP-1
- Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD28 antibody at 1/1500 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-CD28 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control：ICC shows negative staining in THP-1 cells. Anti-CD28 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).