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CD13 Mouse mAb (S-689-37)

Aminopeptidase N,AP-N,hAPN,Alanyl aminopeptidase,Aminopeptidase M (AP-M),Microsomal aminopeptidase,Myeloid plasma membrane glycoprotein CD13,gp150,ANPEP,APN,PEPN

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0441

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Mouse
抗原名称

CD13
分子别名

Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M (AP-M), Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, ANPEP, APN, PEPN
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P15144
克隆号

S-689-37
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
FCM 1:2000
ICC 1:500

应用	稀释度
WB	1:1000
FCM	1:2000
ICC	1:500

背景介绍

Aminopeptidase N (also called CD13) is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. CD13 is also used by some viruses as a receptor to which these viruses bind to and then enter cells. It is a receptor for human coronavirus 229E, feline coronavirus serotype II (FCoV-II), TGEV, PEDV, canine coronavirus genotype II (CCoV-II) as well as several Deltacoronaviruses.

免疫印迹
- WB result of CD13 Mouse mAb
  Primary antibody: CD13 Mouse mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Lane 3: PANC-1 whole cell lysate 20 µg
  Negative control: HEK-293 whole cell lysate
  Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 110 kDa
  Observed MW: 150~160 kDa
流式分析
- Flow cytometric analysis of HEK293 (Human embryonic kidney epithelial cell, left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling CD13 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: HEK293
免疫细胞化学
- ICC shows positive staining in THP-1 cells. Anti-CD13 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control：ICC shows negative staining in HEK293 cells. Anti-CD13 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).