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Fer Recombinant Rabbit mAb (S-R303)

Tyrosine-protein kinase Fer,Feline encephalitis virus-related kinase FER,Fujinami poultry sarcoma/Feline sarcoma-related protein Fer,Proto-oncogene c-Fer,Tyrosine kinase 3,p94-Fer,TYK3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0432

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Fer
分子别名

Tyrosine-protein kinase Fer, Feline encephalitis virus-related kinase FER, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Proto-oncogene c-Fer, Tyrosine kinase 3, p94-Fer, TYK3
细胞定位

Cytoplasm, Nucleus, Cell membrane
Accession

P16591
克隆号

S-R303
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICFCM 1:50 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICFCM	1:50	Hu
ICC	1:500	Hu

背景介绍

Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.

免疫印迹
- WB result of Fer Rabbit mAb
  Primary antibody: Fer Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Lane 4: LNCaP whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 95 kDa
  Observed MW: 95 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of Fer Rabbit mAb
  Primary antibody: Fer Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 95 kDa
  Observed MW: 95 kDa
- WB result of Fer Rabbit mAb
  Primary antibody: Fer Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 5 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 95 kDa
  Observed MW: 95 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Fer antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-Fer antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).