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CHAF1b Recombinant Rabbit mAb (S-R316)

Chromatin assembly factor 1 subunit B,CAF-1 subunit B,Chromatin assembly factor I p60 subunit (CAF-I 60 kDa subunit,CAF-I p60),M-phase phosphoprotein 7,CAF1A,CAF1P60,MPHOSPH7,MPP7

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0430

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

CHAF1b
分子别名

Chromatin assembly factor 1 subunit B, CAF-1 subunit B, Chromatin assembly factor I p60 subunit (CAF-I 60 kDa subunit; CAF-I p60); M-phase phosphoprotein 7, CAF1A, CAF1P60, MPHOSPH7, MPP7
细胞定位

Cytoplasm, Nucleus
Accession

Q13112
克隆号

S-R316
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:500
ICC 1:500
ICFCM 1:500
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:500
ICC	1:500
ICFCM	1:500
IP	1:50

背景介绍

Chromatin assembly factor I (CAF-1) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1b corresponds to the p60 subunit and is required for chromatin assembly after replication. It is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

免疫印迹
- WB result of CHAF1b Rabbit mAb
  Primary antibody: CHAF1b Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: Caco-2 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 61 kDa
  Observed MW: 61 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling CHAF1b antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- CHAF1b Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CHAF1b in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using CHAF1b Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: CHAF1b Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 61 kDa
  Observed MW: 61 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CHAF1b antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-CHAF1b antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).