CD45 Mouse mAb (S-839-3)
CD45,PTPRC,GP180,LCA,T200,CD9 partner 1,CD9P-1,Glu-Trp-Ile EWI motif-containing protein F,EWI-F,Prostaglandin F2-alpha receptor regulatory protein,Prostaglandin F2-alpha receptor-associated protein,Protein-tyrosine phosphatase eta,R-PTP-eta,Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen (L-CA)
货号： S0B0427
- 价格： ￥600
- 规格：
- 数量：
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宿主来源
Mouse抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P08575克隆号
S-839-3抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM, ICC反应种属 ?
Hu纯化方式
Protein G浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
| FCM | 1:10000 |
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
流式分析
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD45 antibody at 1/10000 dilution (0.01 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: HeLa
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD45 antibody at 1/1000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti-CD45 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in HeLa cells. Anti-CD45 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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