CD45 Mouse mAb (S-839-3)

Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen (L-CA),T200,PTPRC

Datasheet COA

价格￥2,100.00 （供应商现货： 3-5个工作日）

货号 S0B0427

规格

100μl1ml25μl

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Mouse
抗原名称

CD45
分子别名

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P08575
克隆号

S-839-3
抗体类型

Mouse mAb
抗体同种型

IgG1,k
应用

FCM ,
ICC ?
稀释度

应用稀释度

ICC 1:500
FCM 1:10000
反应种属 ?

Hu
纯化方式

Protein G
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

应用	稀释度
ICC	1:500
FCM	1:10000

背景介绍

CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.

流式分析
- Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD45 antibody at 1/10000 dilution (0.01 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: HeLa
- Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD45 antibody at 1/1000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-CD45 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in HeLa cells. Anti-CD45 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).