CD5 Mouse mAb (S-576-35)

T-cell surface glycoprotein CD5,Lymphocyte antigen T1/Leu-1,LEU1

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0425

规格

25μl100μl1ml

数量

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产品规格

应用	稀释度
FCM	1:2000
ICC	1:100

背景介绍

CD5 is a cluster of differentiation expressed on the surface of T cells (various species) and in a subset of murine B cells known as B-1a. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. There is no confirmed ligand for CD5 but there is evidence that CD72, a C-type lectin, may be a ligand or that CD5 may be homophilic, binding CD5 on the surface of other cells.

流式分析
- Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / Molt-4 (Human lymphoblastic leukemia T lymphoblast, Right) cells labelling CD5 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: HeLa
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- ICC shows positive staining in Molt-4 cells. Anti-CD5 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control：ICC shows negative staining in HeLa cells. Anti-CD5 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).