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Atg7 Recombinant Rabbit mAb (S-R302)

Ubiquitin-like modifier-activating enzyme ATG7,ATG12-activating enzyme E1 ATG7,Autophagy-related protein 7 (APG7-like,hAGP7),Ubiquitin-activating enzyme E1-like protein,APG7L

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0424

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

ATG7
分子别名

Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7 (APG7-like; hAGP7), Ubiquitin-activating enzyme E1-like protein, APG7L
细胞定位

Cytoplasm
Accession

O95352
克隆号

S-R302
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB ,
IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:500
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:500
IP	1:50

背景介绍

ATG 7, present in both plant and animal genomes, acts as an essential protein for cell degradation and its recycling. The sequence associates with the ubiquitin- proteasome system, UPS, required for the unique development of an autophagosomal membrane and fusion within cells. During the initiation of autophagy, ATG7 acts like an E-1 enzyme for ubiquitin-like proteins (UBL) such as ATG12 and ATG8. ATG7 helps these UBL proteins in targeting their molecule by binding to them and activating their transfer to an E-2 enzyme. ATG7's role in both of these autophagy-specific UBL systems makes it an essential regulator of autophagosome assembly.

免疫印迹
- WB result of Atg7 Rabbit mAb
  Primary antibody: Atg7 Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: HT-29 whole cell lysate 20 µg
  Negative control: Jurkat whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 77 kDa
  Observed MW: 75 kDa
免疫沉淀
- Atg7 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Atg7 in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using Atg7 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: Atg7 Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 77 kDa
  Observed MW: 75 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Atg7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Atg7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Atg7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Atg7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.