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宿主来源
Rabbit抗原名称
Pembrolizumab免疫原
Purified antibody抗体类型
Polyclonal antibody抗体同种型
IgG纯化方式
Immunogen Affinity浓度
Lot specific* (generally 0.2 to 5 mg/ml)*标记
Unconjugated性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4
储存条件
12 months from date of receipt, -20 to -70 °C as supplied.-80℃ for longer storage.
应用
ELISA
Pembrolizumab, sold under the brand name Keytruda, is a humanized antibody used in cancer immunotherapy that treats melanoma, lung cancer, head and neck cancer, Hodgkin lymphoma, stomach cancer, cervical cancer, and certain types of breast cancer. It is an IgG4 isotype antibody that blocks a protective mechanism of cancer cells and thereby, allows the immune system to destroy them. It targets the programmed cell death protein 1 (PD-1) receptor of lymphocytes. Pembrolizumab was approved for medical use in the United States in 2014. The product is produced from serum of some rabbits immunized with Pembrolizumab. It is specific for Pembrolizumab. This antibody serves as an excellent positive control for Pembrolizumab immunogenicity (ADA) assays.
斑点杂交
Dot blot result of Anti-Pembrolizumab Rabbit pAb
Lane 1: Fab of Pembrolizumab
Lane 2: Pembrolizumab
Lane 3: Human total IgG
Lane 4: Human IgG4
Primary antibody: Anti-Pembrolizumab Rabbit pAb at 1/500 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
(This blot was developed with high sensitivity substrate)
ELISA
ELISA binding of Anti-Pembrolizumab Rabbit pAb with Pembrolizumab and
human lgG /lgG4.
Coating antigen: Pembrolizumab, Human lgG, Human lgG 4, all coat with 0.1 ug/ml.
Anti-Pembrolizumab Rabbit pAb, dilution starts from 1000 ng/ml.
EC50=13.89 ng/ml.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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