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SIRT1 Recombinant Rabbit mAb (S-R297)

NAD-dependent protein deacetylase sirtuin-1,hSIRT1,NAD-dependent protein deacylase sirtuin-1,Regulatory protein SIR2 homolog 1,SIR2-like protein 1 (hSIR2),SIR2L1

货号： S0B0418

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SIRT1

  • 分子别名

    NAD-dependent protein deacetylase sirtuin-1, hSIRT1, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1 (hSIR2), SIR2L1

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    Q96EB6

  • 克隆号

    S-R297

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:500
ICFCM 1:50
背景介绍

  • SIRT1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. SIRT1 is downregulated in cells that have high insulin resistance.[10] Furthermore, SIRT1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

  • 免疫印迹

    • WB result of SIRT1 Rabbit mAb

      Primary antibody: SIRT1 Rabbit mAb at 1/1000 dilution

      Lane 1: HepG2 whole cell lysate 20 µg

      Lane 2: Caco-2 whole cell lysate 20 µg

      Lane 3: HEK-293 whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 82 kDa

      Observed MW: 120 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SIRT1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-SIRT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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