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LDH-C/A Recombinant Rabbit mAb (S-R328)

L-lactate dehydrogenase C chain,Cancer/testis antigen 32 (CT32),LDH testis subunit,LDH-X,LDH3,L-lactate dehydrogenase A chain,Cell proliferation-inducing gene 19 protein,LDH muscle subunit (LDH-M),Renal carcinoma antigen NY-REN-59,PIG19

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0416

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

LDH-C/A
分子别名

L-lactate dehydrogenase C chain, Cancer/testis antigen 32 (CT32), LDH testis subunit, LDH testis subunit, LDH-X, LDH3, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, LDH muscle subunit (LDH-M), Renal carcinoma antigen NY-REN-59, PIG19
细胞定位

Cytoplasm
Accession

P07864, P00338
克隆号

S-R328
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms
IHC-P 1:8000 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms
IHC-P	1:8000	Hu, Ms

背景介绍

Lactate dehydrogenase (LDH) is mainly responsible for catalyzing the conversion of pyruvate into lactate. It is present in almost all living cells. It consists of four subunits forming a tetramer and can form five different subtypes of LDH. Their enzymatic properties are similar, but there are obvious specific differences in tissue distribution. LDH-M and LDH-H subunits are encoded by the LDHA and LDHB genes and can also be expressed as LDHA and LDHB. LDHC is the testis-specific LDH protein.

免疫印迹
- WB result of LDH-C/A Rabbit mAb
  Primary antibody: LDH-C/A Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 34 kDa
- WB result of LDH-C/A Rabbit mAb
  Primary antibody: LDH-C/A Rabbit mAb at 1/2000 dilution
  Lane 1: mouse liver lysate 5 µg
  Lane 2: mouse testis lysate 5 µg
  Negative control: mouse liver lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 34 kDa
  Exposure time: 30 s
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.