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LDH-C/A Recombinant Rabbit mAb (S-R328)

L-lactate dehydrogenase C chain,Cancer/testis antigen 32 (CT32),LDH testis subunit,LDH-X,LDH3,L-lactate dehydrogenase A chain,Cell proliferation-inducing gene 19 protein,LDH muscle subunit (LDH-M),Renal carcinoma antigen NY-REN-59,PIG19

货号： S0B0416

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    LDH-C/A

  • 分子别名

    L-lactate dehydrogenase C chain, Cancer/testis antigen 32 (CT32), LDH testis subunit, LDH testis subunit, LDH-X, LDH3, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, LDH muscle subunit (LDH-M), Renal carcinoma antigen NY-REN-59, PIG19

  • 细胞定位

    Cytoplasm

  • Accession

    P07864, P00338

  • 克隆号

    S-R328

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20°C as supplied

稀释度
应用 稀释度
WB 1:1000-1:2000
IHC-P 1:8000
背景介绍

  • Lactate dehydrogenase (LDH) is mainly responsible for catalyzing the conversion of pyruvate into lactate. It is present in almost all living cells. It consists of four subunits forming a tetramer and can form five different subtypes of LDH. Their enzymatic properties are similar, but there are obvious specific differences in tissue distribution. LDH-M and LDH-H subunits are encoded by the LDHA and LDHB genes and can also be expressed as LDHA and LDHB. LDHC is the testis-specific LDH protein.

  • 免疫印迹

    • WB result of LDH-C/A Rabbit mAb                 

      Primary antibody: LDH-C/A Rabbit mAb at 1/1000 dilution

      Lane 1: HepG2 whole cell lysate 20 µg

      Lane 2: HeLa whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 36 kDa

      Observed MW: 34 kDa

    • WB result of LDH-C/A Rabbit mAb                 

      Primary antibody: LDH-C/A Rabbit mAb at 1/2000 dilution

      Lane 1: mouse liver lysate 5 µg

      Lane 2: mouse testis lysate 5 µg

      Negative control: mouse liver lysate

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 36 kDa

      Observed MW: 34 kDa

      Exposure time: 30 s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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