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eRF3/GSPT1 Recombinant Rabbit mAb (S-R325)

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A,Eukaryotic peptide chain release factor subunit 3a,eRF3a,G1 to S phase transition protein 1 homolog

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0414

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

eRF3/GSPT1
分子别名

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog
细胞定位

Cytoplasm
Accession

P15170
克隆号

S-R325
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
IP 1:50 Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
IP	1:50	Rt

背景介绍

GSPT1 is a protein involved in the termination of translation, a process in which ribosomes synthesize proteins after the transcription of DNA to RNA. There is a demonstrated link between GSPT1 degradation and antitumor activity.

免疫印迹
- WB result of eRF3/GSPT1 Rabbit mAb
  Primary antibody: eRF3/GSPT1 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: PC-3 whole cell lysate 20 µg
  Lane 4: A431 whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Lane 6: SK-OV-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 80 kDa
- WB result of eRF3/GSPT1 Rabbit mAb
  Primary antibody: eRF3/GSPT1 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: Raw 264.7 whole cell lysate 20 µg
  Lane 3: mouse brain lysate 20 µg
  Lane 4: mouse testis lysate 20 µg
  Lane 5: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 80 kDa
- WB result of eRF3/GSPT1 Rabbit mAb
  Primary antibody: eRF3/GSPT1 Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Lane 2: rat testis lysate 20 µg
  Lane 3: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 80 kDa
免疫沉淀
- eRF3/GSPT1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating eRF3/GSPT1 in 0.4 mg PC-12 whole cell lysate.
  Western blot was performed on the immunoprecipitate using eRF3/GSPT1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: PC-12 whole cell lysate 20 µg (Input)
  Lane 2: eRF3/GSPT1 Rabbit mAb IP in PC-12 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in PC-12 whole cell lysate
  Predicted MW: 55 kDa
  Observed MW: 80 kDa
免疫细胞化学
- ICC shows positive staining in PC-3 cells. Anti-eRF3 GSPT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).