CD21 Mouse mAb (S-764-18)

Complement receptor type 2,Cr2,Complement C3d receptor,Epstein-Barr virus receptor (EBV receptor)

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0410

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Mouse
抗原名称

CD21
分子别名

Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor (EBV receptor)
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P20023
克隆号

S-764-18
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?
稀释度

应用稀释度

FCM 1:200
ICC 1:500

应用	稀释度
FCM	1:200
ICC	1:500

背景介绍

CD21 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d.B cells express CD21 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. CD21 interacts with CD19 and on mature B cells forms a complex with CD81 (TAPA-1). The CD21-CD19-CD81 complex is often called the B cell co-receptor complex, because CD21 binds to opsonized antigens through attached C3d (or iC3b or C3dg) when the B-cell receptor binds antigen. This results in the B cell having greatly enhanced response to the antigen. Epstein-Barr virus (EBV) can bind CD21, enabling EBV to enter and infect B cells.

流式分析
- Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, left) / Ramos (Human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: K562
免疫细胞化学
- ICC shows positive staining in Raji cells. Anti-CD21 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control：ICC shows negative staining in K562 cells. Anti-CD21 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mosue IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).