BID Recombinant Rabbit mAb (S-R300)

BH3-interacting domain death agonist,p22 BID (BID)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0408

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

BID
分子别名

BH3-interacting domain death agonist, p22 BID (BID)
细胞定位

Cytoplasm
Accession

P55957
克隆号

S-R300
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC 1:2000
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC	1:2000
IP	1:50	Hu

背景介绍

BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into organelle membranes, primarily the outer mitochondrial membrane. The expression of BID is upregulated by the tumor suppressor p53, and BID has been shown to be involved in p53-mediated apoptosis.

免疫印迹
- WB result of BID Rabbit mAb
  Primary antibody: BID Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 26 kDa
- WB result of BID Rabbit mAb
  Primary antibody: BID Rabbit mAb at 1/1000 dilution
  Lane 1: Raw 264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 26 kDa
- WB result of BID Rabbit mAb
  Primary antibody: BID Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 26 kDa
免疫沉淀
- BID Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating BID in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using BID Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: BID Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 22 kDa
  Observed MW: 26 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.