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BID Recombinant Rabbit mAb (S-R300)

BH3-interacting domain death agonist,p22 BID (BID)

货号： S0B0408

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    BID

  • 分子别名

    BH3-interacting domain death agonist, p22 BID (BID)

  • 细胞定位

    Cytoplasm

  • Accession

    P55957

  • 克隆号

    S-R300

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20°C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC 1:2000
IP 1:50
背景介绍

  • BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into organelle membranes, primarily the outer mitochondrial membrane. The expression of BID is upregulated by the tumor suppressor p53, and BID has been shown to be involved in p53-mediated apoptosis.

  • 免疫印迹

    • WB result of BID Rabbit mAb
      Primary antibody: BID Rabbit mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: THP-1 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 26 kDa

    • WB result of BID Rabbit mAb
      Primary antibody: BID Rabbit mAb at 1/1000 dilution
      Lane 1: Raw 264.7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 26 kDa

    • WB result of BID Rabbit mAb
      Primary antibody: BID Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 26 kDa

  • 免疫沉淀

    • BID Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating BID in 0.4 mg Jurkat whole cell lysate.
      Western blot was performed on the immunoprecipitate using BID Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: Jurkat whole cell lysate 20 µg (Input)
      Lane 2: BID Rabbit mAb IP in Jurkat whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
      Predicted MW: 22 kDa
      Observed MW: 26 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-BID antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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