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ATF-4 Recombinant Rabbit mAb (S-471-111)

cAMP-dependent transcription factor ATF-4,Activating transcription factor 4,Cyclic AMP-responsive element-binding protein 2,cAMP-responsive element-binding protein 2,Tax-responsive enhancer element-binding protein 67,CREB2,TXREB,TaxREB67

货号： S0B0394

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ATF-4

  • 分子别名

    cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; Tax-responsive enhancer element-binding protein 67; CREB2; TXREB; TaxREB67

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cell membrane, Nucleus, Cytoplasm

  • Accession

    P18848

  • 克隆号

    S-471-111

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICC, WB

  • 反应种属 ?

    Hu, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:100
背景介绍

  • ATF-4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. It belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. ATF4 transcription factor is also known to play role in osteoblast differentiation along with RUNX2 and osterix. ATF4 is also involved in the cannabinoid Δ9-tetrahydrocannabinol–induced apoptosis in cancer cells, by the proapoptotic role of the stress protein p8 via its upregulation of the endoplasmic reticulum stress-related genes ATF4, CHOP, and TRB3.

  • 免疫印迹

    • WB result of ATF-4 Rabbit mAb
      Primary antibody: ATF-4 Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 40 µg
      Lane 2: HeLa treated with Tunicamycin (2µg/mL, 8hr) whole cell lysate 40 µg
      Lane 3: HEK-293 whole cell lysate 40 µg
      Lane 4: HEK-293 treated with Tunicamycin (2µg/mL, 8hr) whole cell lysate 40 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 38 kDa
      Observed MW: 50 kDa

    • WB result of ATF-4 Rabbit mAb
      Primary antibody: ATF-4 Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with Tunicamycin (2µg/mL, 8hr) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 38 kDa
      Observed MW: 50 kDa

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells treated with Tunicamycin (2µg/mL, 8hr). Anti- ATF-4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • Negative control：ICC shows weakly positive staining in HeLa cells untreated with Tunicamycin (2μg/mL, 8hr). Anti- ATF-4 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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