NKX6.1 Recombinant Rabbit mAb (S-260-31)

Homeobox protein Nkx-6.1,Homeobox protein NK-6 homolog A,NKX6-1,NKX6A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0390

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

NKX6.1
分子别名

Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A, NKX6-1, NKX6A
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P78426
克隆号

S-260-31
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP

IF ?
稀释度

应用稀释度推荐种属

IHC 1:200-1:500
IF 1:200 Hu
WB 1:1000 Ms
IP 1:50 Ms

应用	稀释度	推荐种属
IHC	1:200-1:500
IF	1:200	Hu
WB	1:1000	Ms
IP	1:50	Ms

背景介绍

Homeobox protein Nkx-6.1 is a protein that in humans is encoded by the NKX6-1 gene. In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes.

免疫印迹
- WB result of NKX6.1 Rabbit mAb
  Primary antibody: NKX6.1 Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Lane 2: MIN6 whole cell lysate 20 µg
  Negative control: RAW 264.7 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 44, 46 kDa
免疫沉淀
- NKX6.1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating NKX6.1 in 0.4 mg β-TC-6 whole cell lysate.
  Western blot was performed on the immunoprecipitate using NKX6.1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: β-TC-6 whole cell lysate 20 µg (Input)
  Lane 2: NKX6.1 Rabbit mAb IP in β-TC-6 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in β-TC-6 whole cell lysate
  Predicted MW: 38 kDa
  Observed MW: 44,46 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human pancreas. Anti-NKX6.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-NKX6.1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human pancreas. Anti-NKX6.1 antibody was used at 1/200 dilution (green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.