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宿主来源
Rabbit抗原名称
CD239分子别名
Basal cell adhesion molecule, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, BCAM, LU, MSK19细胞定位
MembraneAccession
P50895克隆号
S-R284抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 WB 1:1000 IHC 1:500 IP 1:50
Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membrane glycoprotein that in humans is encoded by the BCAM gene. BCAM has also recently been designated CD239 (cluster of differentiation 239). It is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. BCAM has been shown to interact with Laminin, alpha 5. BCAM has also been shown to promote the metastasis of ovarian cancer.
免疫印迹
WB result of CD239 Rabbit mAb
Primary antibody: CD239 Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Negative control: SH-SY5Y whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 85 kDa
(This blot was developed with high sensitivity substrate)
免疫沉淀
CD239 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD239 in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using CD239 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A431 whole cell lysate 20 µg (Input)
Lane 2: CD239 Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 67 kDa
Observed MW: 85 kDa
（This blot was developed with high sensitivity substrate）
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD239 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-CD239 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD239 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD239 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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