CD50 Recombinant Rabbit mAb (S-666-37)

Intercellular adhesion molecule 3,ICAM-3,CDw50,ICAM-R,ICAM3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0381

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD50
分子别名

Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3
免疫原

Synthetic Peptide
细胞定位

Membrane
Accession

P32942
克隆号

S-666-37
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Cz
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC 1:500
IP 1:50

应用	稀释度
WB	1:1000
IHC	1:500
IP	1:50

背景介绍

Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a protein that in humans is encoded by the ICAM3 gene. The protein is constitutively expressed on the surface of leukocytes, which are also called white blood cells and are part of the immune system. ICAM3 mediates adhesion between cells by binding to specific integrin receptors. It plays an important role in the immune cell response through its facilitation of interactions between T cells and dendritic cells, which allows for T cell activation. ICAM3 also mediates the clearance of cells undergoing apoptosis by attracting and binding macrophages, a type of cell that breaks down infected or dying cells through a process known as phagocytosis, to apoptotic cells.

免疫印迹
- WB result of CD50 Rabbit mAb
  Primary antibody: CD50 Rabbit mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: HL-60 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: Ramos whole cell lysate 20 µg
  Negative control: 293T whole cell lysate
  Low expression control: HL-60 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 110~130 kDa
免疫沉淀
- CD50 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD50 in 0.4 mg Ramos whole cell lysate.
  Western blot was performed on the immunoprecipitate using CD50 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Ramos whole cell lysate 20 µg (Input)
  Lane 2: CD50 Rabbit mAb IP in Ramos whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Ramos whole cell lysate
  Predicted MW: 60 kDa
  Observed MW: 110~130 kDa
  （This blot was developed with high sensitivity substrate）
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human mantle cell lymphoma. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.