CD7 Mouse mAb (S-575-58)

T-cell antigen CD7,GP40,T-cell leukemia antigen,T-cell surface antigen Leu-9,TP41

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0380

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Mouse
抗原名称

CD7
分子别名

T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41
免疫原

Recombinant Protein
细胞定位

Membrane
Accession

P09564
克隆号

S-575-58
抗体类型

Mouse mAb
抗体同种型

IgG1,k
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?
稀释度

应用稀释度

ICC 1:500
FCM 1:2000

应用	稀释度
ICC	1:500
FCM	1:2000

背景介绍

CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

流式分析
- Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD7 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately.
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-CD7 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in Raji cells. Anti-CD7 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).