sTfR Recombinant Rabbit mAb (S-612-12)

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货号： S0B0374

Datasheet COA

价格： ￥260
规格：
10μl25μl100μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

sTfR
分子别名

T9, p90, CD71
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P02786
克隆号

S-612-12
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P, ICC, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC	1:2000
ICC	1:50

背景介绍

Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum. This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds. Its molecular mass 190,000 Dalton. Blood testing of the soluble transferrin receptor (sTfR) is used as a measure of functional iron status and the investigation of iron deficiency anemia. Ferritin, a routine investigation for anemia, is an acute-phase reactant, and may be elevated in states of inflammation, thereby falsely indicating that iron stores are adequate. Because sTfR is insensitive to inflammation, it can detect anemia in patients with preexisting inflammatory states, and is particularly useful in distinguishing between the anemia of chronic disease and anemias caused by lack of iron intake.

免疫印迹
- WB result of sTfR Rabbit mAb
  Primary antibody: sTfR Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: HT-1080 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 100, 85 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-sTfR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-sTfR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-sTfR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows clustered cytoplasm staining in HT-1080 cells treated with BFA (5 μg/ml) for 15min. Anti-sTfR antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- Negative control: ICC shows homogeneous cytoplasm staining in HT-1080 cells untreated with BFA (5 μg/ml) for 15min. Anti-sTfR antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).