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O-Linked N-Acetylglucosamine Recombinant Rabbit mAb (S-R256)

O-GlcNAc

价格 ￥882.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0373
规格
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    O-Linked N-Acetylglucosamine

  • 分子别名

    O-GlcNAc

  • 克隆号

    S-R256

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Species Independent

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度
    WB 1:500
    IHC 1:500
    ICC 1:500
背景介绍

  • O-GlcNAc (short for O-linked GlcNAc or O-linked β-N-acetylglucosamine) is a reversible enzymatic post-translational modification that is found on serine and threonine residues of nucleocytoplasmic proteins. The modification is characterized by a β-glycosidic bond between the hydroxyl group of serine or threonine side chains and N-acetylglucosamine (GlcNAc). It has been suggested that apoptosis is regulated by O-GlcNAc. In various cancers, elevated O-GlcNAc levels have been reported to suppress apoptosis. Caspase-3, caspase-8, and caspase-9 have been reported to be modified by O-GlcNAc. Caspase-8 is modified near its cleavage/activation sites; O-GlcNAc modification may block caspase-8 cleavage and activation by steric hindrance. Pharmacological lowering of O-GlcNAc with 5S-GlcNAc accelerated caspase activation while pharmacological raising of O-GlcNAc with thiamet-G inhibited caspase activation.

  • 免疫印迹

    • WB result of O-Linked N-Acetylglucosamine Rabbit mAb
      Primary antibody: O-Linked N-Acetylglucosamine Rabbit mAb at 1/500 dilution
      Lane 1: A431 whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: Multiple kDa
      Observed MW: Multiple kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of O-Linked N-Acetylglucosamine Rabbit mAb
      Primary antibody: O-Linked N-Acetylglucosamine Rabbit mAb at 1/500 dilution
      Lane 1: C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: Multiple kDa
      Observed MW: Multiple kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of O-Linked N-Acetylglucosamine Rabbit mAb
      Primary antibody: O-Linked N-Acetylglucosamine Rabbit mAb at 1/500 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: Multiple kDa
      Observed MW: Multiple kDa
      (This blot was developed with high sensitivity substrate)

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

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