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RRM1 Recombinant Rabbit mAb (S-587-21)

Ribonucleoside-diphosphate reductase large subunit,Ribonucleoside-diphosphate reductase subunit M1,Ribonucleotide reductase large subunit,RR1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0364

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

RRM1
分子别名

Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large subunit, RR1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P23921
克隆号

S-587-21
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:100 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:100	Hu
ICFCM	1:50	Hu

背景介绍

Ribonucleotide reductase large subunit M1 (RRM1) is a rate-limiting enzyme of the DNA synthesis pathway, and is depended on for the conversion of ribonucleotides to dNTPs. Studies have shown that increased levels of RRM1 are associated with resistance to gemcitabine. RRM1 has also been reported to affect disease prognosis of pancreatic cancer. In fact, the meta-analysis for RRM1 demonstrated that high expression of RRM1 was significantly associated with worse overall survival. The clinical impact of RRM1 has been elucidated

免疫印迹
- WB result of RRM1 Rabbit mAb
  Primary antibody: RRM1 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HT-29 whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 90 kDa
  Observed MW: 90 kDa
- WB result of RRM1 Rabbit mAb
  Primary antibody: RRM1 Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 90 kDa
  Observed MW: 90 kDa
- WB result of RRM1 Rabbit mAb
  Primary antibody: RRM1 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 90 kDa
  Observed MW: 90 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling RRM1 antibody at 1/50 (1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HT-29 cells. Anti-RRM1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).