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IMP-3 Recombinant Rabbit mAb (S-439-10)

Insulin-like growth factor 2 mRNA-binding protein 3,IGF2 mRNA-binding protein 3,IGF-II mRNA-binding protein 3,KH domain-containing protein overexpressed in cancer (hKOC),VICKZ family member 3,IGF2BP3,KOC1,VICKZ3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0361
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    IMP-3

  • 分子别名

    Insulin-like growth factor 2 mRNA-binding protein 3, IGF2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, KH domain-containing protein overexpressed in cancer (hKOC), VICKZ family member 3, IGF2BP3, KOC1, VICKZ3

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    O00425

  • 克隆号

    S-439-10

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC 1:200
    ICC 1:500 Hu
    ICFCM 1:500 Hu
背景介绍

  • Insulin-like growth factor-II (IGF-II) messenger RNA (mRNA)-binding protein-3 (IMP-3), also known as K homology domain-containing protein overexpressed in cancer (KOC) and L523S, is a member of the IGF-II mRNA-binding protein (IMP) family, which also includes IMP-1 and IMP-2. IMP-3 is a 580 amino-acid protein encoded by a 4350-bp mRNA transcript produced by a gene located on chromosome 7p11.5. As a translational activator of IGF-II leader-3 mRNA, it is associated with cell proliferation and is considered an oncofetal protein due to its expression during embryogenesis and in some malignancies. Normal adult tissues that express IMP-3 include term placenta, ovary, testis, brain, lymph node germinal centers, and intestinal mucosa. Increased levels of IMP-3 have been identified in pancreatic carcinoma, renal cell carcinoma, germ cell neoplasms, ovarian carcinoma, and extrapulmonary small-cell carcinoma, as well as high-grade neuroendocrine carcinoma, squamous cell carcinoma, and adenocarcinoma of the lung. Additionally, IMP-3 was shown to be a prognostic marker in patients with renal cell carcinoma, with lack of expression in the primary tumor predicting longer metastases-free survival.

  • 免疫印迹

    • WB result of IMP-3 Rabbit mAb
      Primary antibody: IMP-3 Rabbit mAb at 1/1000 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HepG2 whole cell lysate 20 µg
      Negative control: MCF7 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 63 kDa
      Observed MW: 63 kDa

    • WB result of IMP-3 Rabbit mAb
      Primary antibody: IMP-3 Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse placenta lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 63 kDa
      Observed MW: 63 kDa

    • WB result of IMP-3 Rabbit mAb
      Primary antibody: IMP-3 Rabbit mAb at 1/1000 dilution
      Lane 1: rat placenta lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 63 kDa
      Observed MW: 63 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) labelling IMP-3 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: MCF7

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-IMP-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-IMP-3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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