Stat1 Recombinant Rabbit mAb (S-559-91)

Signal transducer and activator of transcription 1-alpha/beta,Transcription factor ISGF-3 components p91/p84

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0358

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Stat1
分子别名

Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84
细胞定位

Cytoplasm, Nucleus
克隆号

S-559-91
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ChIP ,
WB ,
IP
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied

稀释度

应用	稀释度
WB	1:1000
IP	1:50
ChIP	1:20-1:50

背景介绍

Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. It is a member of the STAT protein family. All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Interleukin 6 (IL-6), or IL-27.

免疫印迹
- WB result of Stat1 Rabbit mAb
  Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 5 µg
  Lane 2: HeLa whole cell lysate 5 µg
  Lane 3: MCF7 whole cell lysate 5 µg
  Lane 4: A549 whole cell lysate 5 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 84, 91 kDa
- WB result of Stat1 Rabbit mAb
  Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse heart lysate 5 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 84, 91 kDa
- WB result of Stat1 Rabbit mAb
  Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
  Lane 1: rat heart lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 91 kDa
免疫沉淀
- Stat1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Stat1 in 0.4 mg 293T whole cell lysate.
  Western blot was performed on the immunoprecipitate using Stat1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: 293T whole cell lysate 20 µg (Input)
  Lane 2: Stat1 Rabbit mAb IP in 293T whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
  Predicted MW: 87 kDa
  Observed MW: 84, 91 kDa
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa+IFN-α (50 ng/ml, 30 min) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used Stat1 Recombinant Rabbit mAb (S-559-91) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  
  qPCR showed the enrichment of IRF1, TAP1 and SAT-α in Stat1 Recombinant
  Rabbit mAb (S-559-91)-immunoprecipitated sample.