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Rabbit抗原名称
IDO1/Indoleamine 2,3-dioxygenase分子别名
Indoleamine-pyrrole 2,3-dioxygenase, IDO, INDO免疫原
Recombinant Protein细胞定位
CytoplasmAccession
P14902克隆号
S-259-62抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC
ICFCM
ICC ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC 1:200-1:1000 ICC 1:100 ICFCM 1:100
Indoleamine-pyrrole 2,3-dioxygenase (IDO or INDO) is a heme-containing enzyme physiologically expressed in a number of tissues and cells, such as the small intestine, lungs, female genital tract or placenta. IDO is an important part of the immune system and plays a part in natural defense against various pathogens. It is produced by the cells in response to inflammation and has an immunosuppressive function because of its ability to limit T-cell function and engage mechanisms of immune tolerance. Emerging evidence suggests that IDO becomes activated during tumor development, helping malignant cells escape eradication by the immune system. Expression of IDO has been described in a number of types of cancer, such as acute myeloid leukemia, ovarian cancer or colorectal cancer.
免疫印迹
WB result of IDO1/Indoleamine 2,3-dioxygenase Rabbit mAb
Primary antibody: IDO1/Indoleamine 2,3-dioxygenase Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with IFN-γ (50ng/ml,16 hr) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 40 kDaWB result of IDO1/Indoleamine 2,3-dioxygenase Rabbit mAb
Primary antibody: IDO1/Indoleamine 2,3-dioxygenase Rabbit mAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 40 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells, treated with IFN-γ (50 ng/ml, 16 hr) (Red) or untreated (Green), labeling IDO1/Indoleamine 2, 3-dioxygenase at 1/100 dilution (0.1 μg) compared with a rabbit monoclonal IgG isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-IDO1/Indoleamine 2,3-dioxygenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-IDO1/Indoleamine 2,3-dioxygenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-IDO1/Indoleamine 2,3-dioxygenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-IDO1/Indoleamine 2,3-dioxygenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti-IDO1/Indoleamine 2,3-dioxygenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells, treated with IFN-γ (50 ng/ml, 16 hr). Anti-IDO/Indoleamine 2,3-dioxygenase Rabbit antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
Negative control: ICC shows negative staining in HeLa cells, untreated with IFN-γ (50 ng/ml, 16 hr). Anti- IDO/Indoleamine 2,3-dioxygenase Rabbit antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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