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别名
Rabbit IgJ,Rabbit Immunoglobulin J chain,Rabbit JCHAIN,Rabbit IGCJ,Immunoglobulin J chain,Joining chain of multimeric IgA and IgM,JCHAIN,IGCJ宿主来源
Rabbit抗原名称
IGJ免疫原
Recombinant Protein细胞定位
SecretedAccession
P01591克隆号
S-342-48抗体类型
Recombinant mAb应用
IHC-P, WB, IP反应种属 ?
Hu纯化方式
Protein A浓度
0.25 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:500 |
| IP | 1:25 |
| IHC-P | 1:4000 |
The Joining (J) chain is a protein component that links monomers of antibodies IgM and IgA to form polymeric antibodies capable of secretion. The J chain regulates the multimerization of IgM and IgA in mammals. When expressed in cells, it favors the formation of a pentameric IgM and an IgA dimer. The J chain is incorporated late in the formation of IgM polymers and thermodynamically favors the formation of pentamers as opposed to hexamers. The J chain facilitates IgA dimerization by linking two monomer secretory tails. Structurally, the J chain joins two antibody monomers asymmetrically by forming intermolecular disulfide bonds and bringing hydrophobic β-sandwiches on each molecule together.
免疫印迹
WB result of IGJ Rabbit mAb
Primary antibody: IGJ Rabbit mAb at 1/500 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 23 kDa
免疫沉淀
IGJ Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating IGJ in 0.4 mg Daudi whole cell lysate.
Western blot was performed on the immunoprecipitate using IGJ Rabbit mAb at 1/500 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: Daudi whole cell lysate 20 µg (Input)
Lane 2: IGJ Rabbit mAb IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
Predicted MW: 18 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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