S-RMab^® IGJ Recombinant Rabbit mAb (S-342-48) STAR

Immunoglobulin J chain,Joining chain of multimeric IgA and IgM,JCHAIN,IGCJ

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0350

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

IGJ
分子别名

Immunoglobulin J chain, Joining chain of multimeric IgA and IgM, JCHAIN, IGCJ
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P01591
克隆号

S-342-48
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:500
IP 1:25
IHC-P 1:4000

应用	稀释度
WB	1:500
IP	1:25
IHC-P	1:4000

背景介绍

The Joining (J) chain is a protein component that links monomers of antibodies IgM and IgA to form polymeric antibodies capable of secretion. The J chain regulates the multimerization of IgM and IgA in mammals. When expressed in cells, it favors the formation of a pentameric IgM and an IgA dimer. The J chain is incorporated late in the formation of IgM polymers and thermodynamically favors the formation of pentamers as opposed to hexamers. The J chain facilitates IgA dimerization by linking two monomer secretory tails. Structurally, the J chain joins two antibody monomers asymmetrically by forming intermolecular disulfide bonds and bringing hydrophobic β-sandwiches on each molecule together.

免疫印迹
- WB result of IGJ Rabbit mAb
  Primary antibody: IGJ Rabbit mAb at 1/500 dilution
  Lane 1: Daudi whole cell lysate 20 µg
  Lane 2: Ramos whole cell lysate 20 µg
  Lane 3: Raji whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 23 kDa
免疫沉淀
- IGJ Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating IGJ in 0.4 mg Daudi whole cell lysate.
  Western blot was performed on the immunoprecipitate using IGJ Rabbit mAb at 1/500 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Daudi whole cell lysate 20 µg (Input)
  Lane 2: IGJ Rabbit mAb IP in Daudi whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
  Predicted MW: 18 kDa
  Observed MW: 23 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-IGJ antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.