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Caspase-8 Recombinant Rabbit mAb (S-R257)

CASP-8,Apoptotic cysteine protease,Apoptotic protease Mch-5,CAP4,FADD-homologous ICE/ced-3-like protease,FADD-like ICE,FLICE,ICE-like apoptotic protease 5,MORT1-associated ced-3 homolog,MACH,MCH5

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0345

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Caspase-8
分子别名

CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, MCH5
细胞定位

Nucleus, Cytoplasm
Accession

Q14790
克隆号

S-R257
抗体类型

Recombinant mAb
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

Caspase-8 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.

免疫印迹
- WB result of Caspase-8 Rabbit mAb
  Primary antibody: Caspase-8 Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: THP-1 whole cell lysate 20 µg
  Lane 4: HCT 116 whole cell lysate 10 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 56 kDa
- WB result of Caspase-8 Rabbit mAb
  Primary antibody: Caspase-8 Rabbit mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Lane 2: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 56 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Caspase-8 antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-Caspase-8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).