TSHB Recombinant Rabbit mAb (S-537-52)

Thyrotropin subunit beta,Thyroid-stimulating hormone subunit beta (TSH-B,TSH-beta),Thyrotropin beta chain

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0343

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

TSHB
分子别名

Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta (TSH-B; TSH-beta), Thyrotropin beta chain
免疫原

Synthetic Peptide
Accession

P01222
克隆号

S-537-52
抗体类型

Recombinant mAb
反应种属 ?

Hu, Rt
预测反应种属
(反应种属缩写表)

Ct, Dg, Hr, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

IF ?
稀释度

应用稀释度推荐种属

IHC 1:2000
IF 1:200 Hu

应用	稀释度	推荐种属
IHC	1:2000
IF	1:200	Hu

背景介绍

Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit (TSHA) and a unique beta subunit (this protein) that confers specificity.

免疫组化
- IHC shows positive staining in paraffin-embedded rat pituitary. Anti-TSHB antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pituitary. Anti-TSHB antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-TSHB antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human pituitary. Anti-TSHB antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.