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MED4 Recombinant Rabbit mAb (S-R258)

Mediator of RNA polymerase II transcription subunit 4,Activator-recruited cofactor 36 kDa component (ARC36),Mediator complex subunit 4,TRAP/SMCC/PC2 subunit p36 subunit,Vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36),ARC36,DRIP36,VDRIP,HSPC126

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0342

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MED4
分子别名

Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component (ARC36), Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), ARC36, DRIP36, VDRIP, HSPC126
细胞定位

Nucleus
Accession

Q9NPJ6
克隆号

S-R258
抗体类型

Recombinant mAb
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu

背景介绍

Mediator of RNA polymerase II transcription subunit 4 also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) is a protein that in humans is encoded by the MED4 gene. The protein encoded by this gene is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner.

免疫印迹
- WB result of MED4 Rabbit mAb
  Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 36 kDa
- WB result of MED4 Rabbit mAb
  Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 36 kDa
- WB result of MED4 Rabbit mAb
  Primary antibody: MED4 Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 36 kDa
免疫沉淀
- MED4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating MED4 in 0.4 mg HepG2 whole cell lysate.
  Western blot was performed on the immunoprecipitate using MED4 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HepG2 whole cell lysate 20 µg (Input)
  Lane 2: MED4 Rabbit mAb IP in HepG2 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
  Predicted MW: 30 kDa
  Observed MW: 36 kDa
  （This blot was developed with high sensitivity substrate）