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HLA-DPB1 Recombinant Rabbit mAb (S-R263)

HLA class II histocompatibility antigen DP beta 1 chain,HLA class II histocompatibility antigen DP(W4) beta chain,MHC class II antigen DPB1,HLA-DP1B,HLA DPB1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0340

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

HLA-DPB1
分子别名

HLA class II histocompatibility antigen DP beta 1 chain, HLA class II histocompatibility antigen DP(W4) beta chain, MHC class II antigen DPB1, HLA-DP1B, HLA DPB1
细胞定位

Cell membrane, Endoplasmic reticulum membrane
Accession

P04440
克隆号

S-R263
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC 1:500-1:2000
ICC 1:500
ICFCM 1:500

应用	稀释度
WB	1:1000
IP	1:50
IHC	1:500-1:2000
ICC	1:500
ICFCM	1:500

背景介绍

HLA class II histocompatibility antigen, DP(W2) beta chain is a protein that in humans is encoded by the HLA-DPB1 gene. HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins.

免疫印迹
- WB result of HLA-DPB1 Rabbit mAb
  Primary antibody: HLA-DPB1 Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: Daudi whole cell lysate 20 µg
  Lane 3: Raji whole cell lysate 20 µg
  Lane 4: Ramos whole cell lysate 20 µg
  Lane 5: HuT 78 whole cell lysate 20 µg
  Negative control: Jurkat whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
流式分析
- Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling HLA-DPB1 antibody at 1/500 (0.1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti –Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- HLA-DPB1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating HLA-DPB1 in 0.2 mg Raji whole cell lysate.
  Western blot was performed on the immunoprecipitate using HLA-DPB1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Raji whole cell lysate 20 µg (Input)
  Lane 2: HLA-DPB1 Rabbit mAb IP in Raji whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Raji whole cell lysate
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human spleen. Anti-HLA-DPB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-HLA-DPB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-HLA-DPB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-HLA-DPB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-HLA-DPB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-HLA-DPB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HLA-DPB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Raji cells. Anti-HLA-DPB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in Jurkat cells. Anti-HLA-DPB1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).