p14ARF Recombinant Rabbit mAb (S-R244)

Tumor suppressor ARF,Alternative reading frame (ARF),Cyclin-dependent kinase inhibitor 2A,CDKN2A,MLM

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0338

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

p14ARF
分子别名

Tumor suppressor ARF, Alternative reading frame (ARF), Cyclin-dependent kinase inhibitor 2A, CDKN2A, MLM
细胞定位

Nucleus
Accession

Q8N726
克隆号

S-R244
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC 1:100
ICFCM 1:500
ICC 1:500
IP 1:50

应用	稀释度
WB	1:1000
IHC	1:100
ICFCM	1:500
ICC	1:500
IP	1:50

背景介绍

p14ARF (also called ARF tumor suppressor, ARF, p14ARF) is an alternate reading frame protein product of the CDKN2A locus (i.e. INK4a/ARF locus). p14ARF is induced in response to elevated mitogenic stimulation, such as aberrant growth signaling from MYC and Ras (protein). It accumulates mainly in the nucleolus where it forms stable complexes with NPM or Mdm2. These interactions allow p14ARF to act as a tumor suppressor by inhibiting ribosome biogenesis or initiating p53-dependent cell cycle arrest and apoptosis, respectively.

免疫印迹
- WB result of p14ARF Rabbit mAb
  Primary antibody: p14ARF Rabbit mAb at 1/1000 dilution
  Lane 1: SK-OV-3 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: Saos-2 whole cell lysate 20 µg
  Negative control: SK-OV-3 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 14 kDa
  Observed MW: 14 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SK-OV-3 (Human ovarian cancer epithelial cell, left) / HeLa (Human cervix adenocarcinoma epithelial cell, right) cells labelling p14ARF antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: SK-OV-3
免疫沉淀
- p14ARF Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p14ARF in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using p14ARF Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: p14ARF Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 14 kDa
  Observed MW: 14 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-p14ARF antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-p14ARF antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-p14ARF antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-p14ARF antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-p14ARF antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
- Negative control：ICC shows negative staining in SK-OV-3 cells. Anti-p14ARF antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).