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p16 Recombinant Rabbit mAb (S-303-1)

Cyclin-dependent kinase inhibitor 2A,Cyclin-dependent kinase 4 inhibitor A (CDK4I),Multiple tumor suppressor 1 (MTS-1),p16-INK4a (p16-INK4,p16INK4A),CDKN2A,CDKN2,MTS1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0336

规格

25μl1ml100μl

数量

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产品规格

宿主来源

Rabbit
抗原名称

p16
分子别名

Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A (CDK4I), Multiple tumor suppressor 1 (MTS-1), p16-INK4a (p16-INK4; p16INK4A), CDKN2A, CDKN2, MTS1
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Nucleus
Accession

P42771
克隆号

S-303-1
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
ICC ? ,
WB ,
IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
ICC 1:100
ICFCM 1:500

应用	稀释度
WB	1:1000
IP	1:50
ICC	1:100
ICFCM	1:500

背景介绍

p16 (also known as p16INK4a, cyclin-dependent kinase inhibitor 2A, CDKN2A, multiple tumor suppressor 1 and numerous other synonyms), is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby acting as a tumor suppressor. p16 can be used as a biomarker to improve the histological diagnostic accuracy of grade 3 cervical intraepithelial neoplasia (CIN). p16 is also implicated in the prevention of melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, vulvar cancer and esophageal cancer.

免疫印迹
- WB result of p16 Rabbit mAb
  Primary antibody: p16 Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: HEK-293 whole cell lysate 20 µg
  Negative control: MCF7 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 16 kDa
  Observed MW: 16 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling p16 antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- p16 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p16 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using p16 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: p16 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 16 kDa
  Observed MW: 16 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-p16 antibody was used at 1/100 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Green).