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TACSTD2/TROP2 Recombinant Rabbit mAb (S-R237)

Tumor-associated calcium signal transducer 2,Cell surface glycoprotein Trop-2,Membrane component chromosome 1 surface marker 1,Pancreatic carcinoma marker protein GA733-1,GA733-1,M1S1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0323
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产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    TACSTD2/TROP2

  • 分子别名

    Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1, GA733-1, M1S1

  • 细胞定位

    Membrane

  • Accession

    P09758

  • 克隆号

    S-R237

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB ,
    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Rt
    IHC 1:500
    IP 1:35 Hu
背景介绍

  • Tumor-associated calcium signal transducer 2, also known as Trop-2 and as epithelial glycoprotein-1 antigen (EGP-1), is a protein that in humans is encoded by the TACSTD2 gene. Trop-2 expression was originally described in trophoblasts (placenta) and fetal tissues (e.g., lung). Later, its expression was also described in the normal stratified squamous epithelium of the skin, uterine cervix, esophagus, and tonsillar crypts. Trop-2 plays a role in tumor progression by actively interacting with several key molecular signaling pathways traditionally associated with cancer development and progression. Aberrant overexpression of Trop-2 has been described in several solid cancers, such as colorectal, renal, lung, and breast cancers. Trop-2 expression has also been described in some rare and aggressive malignancies, e.g., salivary duct, anaplastic thyroid, uterine/ovarian, and neuroendocrine prostate cancers.

  • 免疫印迹

    • WB result of TACSTD2/TROP2 Rabbit mAb
      Primary antibody: TACSTD2/TROP2 Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: HCT 116 whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 37, 50 kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of TACSTD2/TROP2 Rabbit mAb
      Primary antibody: TACSTD2/TROP2 Rabbit mAb at 1/1000 dilution
      Lane 1: rat skin lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 37, 50 kDa
      (This blot was developed with high sensitivity substrate)

  • 免疫沉淀

    • TACSTD2/TROP2 Rabbit mAb at 1/35 dilution (1 µg) immunoprecipitating TACSTD2/TROP2 in 0.28 mg MCF-7 whole cell lysate.
      Western blot was performed on the immunoprecipitate using TACSTD2/TROP2 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1:MCF-7 whole cell lysate 20 µg (Input)
      Lane 2: TACSTD2/TROP2 Rabbit mAb IP in MCF-7 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in MCF-7 whole cell lysate
      Predicted MW: 36 kDa
      Observed MW: 37,50 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human esophagus. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostate. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic carcinoma. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid carcinoma. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat skin. Anti-TACSTD2/TROP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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