S6 Ribosomal Protein Recombinant Rabbit mAb (S-479-1)

Small ribosomal subunit protein eS6,40S ribosomal protein S6,Phosphoprotein NP33,RPS6

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0320

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

S6 Ribosomal Protein
分子别名

Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33, RPS6
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P62753
克隆号

S-479-1
抗体类型

Recombinant mAb
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Av, Bv, Rb, Xe, Fs
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC 1:500
ICFCM 1:5000 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC	1:500
ICFCM	1:5000	Hu

背景介绍

Ribosomal protein S6 (rpS6 or eS6) is a component of the 40S ribosomal subunit and is therefore involved in translation. Mouse model studies have shown that phosphorylation of eS6 is involved in the regulation of cell size, cell proliferation, and glucose homeostasis. tudies show that the p70 ribosomal protein S6 kinases (S6K1 and S6K2) and p90 ribosomal protein S6 kinases (RSK) both phosphorylate eS6 and that S6K1 and S6K2 predominate this function. Pathways leading to the induction of human eS6 phosphorylation have been found to enhance IL-8 protein synthesis. This mechanism is dependent on A/U-rich proximal sequences (APS) found in the 3'UTR of IL-8 immediately after the stop codon.

免疫印迹
- WB result of S6 Ribosomal Protein Rabbit mAb
  Primary antibody: S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
- WB result of S6 Ribosomal Protein Rabbit mAb
  Primary antibody: S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
- WB result of S6 Ribosomal Protein Rabbit mAb
  Primary antibody: S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling S6 Ribosomal Protein at 1/5000 dilution (0.01 μg) / (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- S6 Ribosomal Protein Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating S6 Ribosomal Protein in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: S6 Ribosomal Protein Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.