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宿主来源
Rabbit抗原名称
FGFR2分子别名
Fibroblast growth factor receptor 2, K-sam (KGFR), Keratinocyte growth factor receptor, CD332, BEK, KSAM细胞定位
Cell membraneAccession
P21802克隆号
S-R239抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
FCM
ICC ?
稀释度
应用 稀释度 ICC 1:500 FCM 1:500
Fibroblast growth factor receptor 2 (FGFR2) is one of four FGFRs that encode a transmembrane receptor tyrosine kinase. FGFR2 signaling is associated with the proliferation, migration, and angiogenesis of carcinomas. FGFR2-overexpressed cancer might be a therapeutic target. Several FGFR2 inhibitors have been developed for the treatment of cancer patients with enhanced expression of FGFR2 signaling7FGFR2 has two isoforms, i.e., the IIIb type and the IIIc type based on the alternative splicing within the C-terminal half of the third Ig loop (D3) in the extracellular FGF binding domain, which are alternatively spliced by exon 8 and by exon 9, respectively. The spliced isoforms differ in binding ligands: FGFR2IIIb is a high-affinity receptor for FGF1, -3, -7, -10, and -22, whereas FGFR2IIIc binds FGF1, -2, -4, -6, -8, -9 -17 and-18. The FGFR2IIIb isoform is expressed mainly in epithelial cells, and it preferentially binds secreted FGF ligands from adjacent mesenchymal cells. In contrast, the FGFR2IIIc isoform is preferentially expressed in mesenchymal cells and usually binds ligands secreted from the adjacent epithelial cells.
流式分析
Flow cytometric analysis of SW620 (Human colorectal adenocarcinoma epithelial cell, left) / KATO III (human gastric carcinoma, right) cells labelling FGFR2 antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: SW620
免疫细胞化学
ICC shows positive staining in KATO Ⅲ cells. Anti-FGFR2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
Negative control: ICC shows negative staining in SW620 cells. Anti-FGFR2 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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