Human IgG3 F(c) Recombinant Rabbit mAb, PBS Only (S-459-75)

Immunoglobulin heavy constant gamma 3,HDC,Heavy chain disease protein,Ig gamma-3 chain C region,IGHG3

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货号 S0B0312P

规格

100μg1mg

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Human IgG3 F(c)
分子别名

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3
免疫原

Synthetic Peptide
细胞定位

Secreted, Cell membrane
Accession

P01860
克隆号

S-459-75
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS pH7.4
储存条件

2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
应用

IHC-P ?

ELISA

WB
稀释度

应用稀释度

WB 1:1000
IHC-P 1:2000

应用	稀释度
WB	1:1000
IHC-P	1:2000

背景介绍

The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].

免疫印迹
- WB result of Human IgG3 F(c) Rabbit mAb
  Primary antibody: Human IgG3 F(c) Rabbit mAb at 1/1000 dilution
  Lane 1: Human serum lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
- WB result of Human IgG3 F(c) Rabbit mAb
  Primary antibody: Human IgG3 F(c) Rabbit mAb at 1/1000 dilution
  Lane 1: IgG1 F(c) recombinant protein lysate 10 ng
  Lane 2: IgG2 F(c) recombinant protein lysate 10 ng
  Lane 3: IgG3 F(c) recombinant protein lysate 10 ng
  Lane 4: IgG4 F(c) recombinant protein lysate 10 ng
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: IgG1 F(c)、IgG2 F(c)、IgG4 F(c): 27kDa; IgG3 F(c): 33kDa
  Observed MW: IgG3 F(c): 40kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.