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Human IgG1 F(c) Recombinant Rabbit mAb, PBS Only (S-447-20)

Immunoglobulin heavy constant gamma 1,Ig gamma-1 chain C region,Ig gamma-1 chain C region EU,Ig gamma-1 chain C region KOL,Ig gamma-1 chain C region NIE,IGHG1

Datasheet COA

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货号 S0B0311P

规格

100μg1mg

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Human IgG1 F(c)
分子别名

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
免疫原

Synthetic Peptide
细胞定位

Secreted, Cell membrane
Accession

P01857
克隆号

S-447-20
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS pH7.4
储存条件

2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
应用

IHC-P ?

ELISA

WB

IF ?
稀释度

应用稀释度

WB 1:500-1:10000
IHC-P 1:1000
IF 1:1000

应用	稀释度
WB	1:500-1:10000
IHC-P	1:1000
IF	1:1000

背景介绍

The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].

免疫印迹
- WB result of Human IgG1 F(c) Rabbit mAb
  Primary antibody: Human IgG1 F(c) Rabbit mAb at 1/10000 dilution
  Lane 1: Human serum lysate 5 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 52 kDa
- WB result of Human IgG1 F(c) Rabbit mAb
  Primary antibody: Human IgG1 F(c) Rabbit mAb at 1/500 dilution
  Lane 1: Human IgG recombinant protein lysate 20 ng
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 55 kDa
- WB result of Human IgG1 F(c) Rabbit mAb
  Primary antibody: Human IgG1 F(c) Rabbit mAb at 1/500 dilution
  Lane 1: IgG2 F(c) recombinant protein lysate 10 ng
  Lane 2: IgG3 F(c) recombinant protein lysate 10 ng
  Lane 3: IgG4 F(c) recombinant protein lysate 10 ng
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: IgG2 F(c)、IgG4 F(c): 27kDa; IgG3 F(c): 33kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG1 F(c) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.