热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/9）
申请试用

ACSL4/FACL4 Recombinant Rabbit mAb (S-461-6)

Long-chain-fatty-acid--CoA ligase 4,Arachidonate--CoA ligase,Long-chain acyl-CoA synthetase 4 (LACS 4),ACS4

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0308
规格
数量
收藏 分享

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ACSL4/FACL4

  • 分子别名

    Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4 (LACS 4), ACS4

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Endoplasmic reticulum membrane, Mitochondrion outer membrane, Cell membrane

  • Accession

    O60488

  • 克隆号

    S-461-6

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM ,
    IHC-P ? ,
    ICC ? ,
    WB ,
    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Hu
    IHC 1:200-1:500
    ICC 1:500 Hu
    ICFCM 1:500 Hu
背景介绍

  • ACSL4 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the intellectual disability or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

  • 免疫印迹

    • WB result of ACSL4/FACL4 Rabbit mAb
      Primary antibody: ACSL4/FACL4 Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: 293T whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 79 kDa
      Observed MW: 79 kDa

    • WB result of ACSL4/FACL4 Rabbit mAb
      Primary antibody: ACSL4/FACL4 Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 79 kDa
      Observed MW: 79 kDa

    • WB result of ACSL4/FACL4 Rabbit mAb
      Primary antibody: ACSL4/FACL4 Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 79 kDa
      Observed MW: 79 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling ACSL4/FACL4 antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • ACSL4/FACL4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ACSL4/FACL4 in 0.4 mg HepG2 whole cell lysate.
      Western blot was performed on the immunoprecipitate using ACSL4/FACL4 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HepG2 whole cell lysate 20 µg (Input)
      Lane 2: ACSL4/FACL4 Rabbit mAb IP in HepG2 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
      Predicted MW: 79 kDa
      Observed MW: 79 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-ACSL4/FACL4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-ACSL4/FACL4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ACSL4/FACL4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-ACSL4/FACL4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

引用文献(1)

评论(0)